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代欢欢, 山雨思, 辛正琦, 何潇, 吴能表. 外源甜菜碱对盐胁迫下颠茄生理特性及托品烷类生物碱含量的影响[J]. 植物科学学报, 2020, 38(3): 400-409. DOI: 10.11913/PSJ.2095-0837.2020.30400
引用本文: 代欢欢, 山雨思, 辛正琦, 何潇, 吴能表. 外源甜菜碱对盐胁迫下颠茄生理特性及托品烷类生物碱含量的影响[J]. 植物科学学报, 2020, 38(3): 400-409. DOI: 10.11913/PSJ.2095-0837.2020.30400
Dai Huan-Huan, Shan Yu-Si, Xin Zheng-Qi, He Xiao, Wu Neng-Biao. Effects of exogenous betaine on physiological characteristics and tropidine alkaloid content in Atropa belladonna L. under NaCl stress[J]. Plant Science Journal, 2020, 38(3): 400-409. DOI: 10.11913/PSJ.2095-0837.2020.30400
Citation: Dai Huan-Huan, Shan Yu-Si, Xin Zheng-Qi, He Xiao, Wu Neng-Biao. Effects of exogenous betaine on physiological characteristics and tropidine alkaloid content in Atropa belladonna L. under NaCl stress[J]. Plant Science Journal, 2020, 38(3): 400-409. DOI: 10.11913/PSJ.2095-0837.2020.30400

外源甜菜碱对盐胁迫下颠茄生理特性及托品烷类生物碱含量的影响

Effects of exogenous betaine on physiological characteristics and tropidine alkaloid content in Atropa belladonna L. under NaCl stress

  • 摘要: 以颠茄(Atropa belladonna L.)幼苗为材料,通过人工模拟盐胁迫条件,探讨在100 mmol/L盐胁迫下喷施不同浓度(20 mmol/L和40 mmol/L)的甜菜碱(GB)溶液对颠茄幼苗生理特性及托品烷类生物碱含量的影响。结果显示,外源甜菜碱增强了颠茄叶片的光合作用,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性均有不同程度的提高;丙二醛(MDA)含量显著下降,硝态氮、可溶性蛋白、游离氨基酸含量及硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸脱氢酶(GDH)活性显著升高。颠茄次生代谢途径中的前体氨基酸(鸟氨酸和精氨酸)的含量以及托品烷类生物碱(莨菪碱和东莨菪碱)的含量也明显升高。研究结果表明100 mmol/L盐胁迫处理对颠茄的生长产生了抑制作用,而20 mmol/L甜菜碱处理能较好地缓解这种伤害。

     

    Abstract: In this study, Atropa belladonna L. seedlings were sprayed with different concentrations (20 mmol/L and 40 mmol/L) of betaine (glycine betaine, GB) under artificial simulated 100 mmol/L salt stress to investigate the effects on physiological characteristics and tropane alkaloid content in Atropa belladonna L. Compared with NaCl stress treatment, exogenous betaine enhanced the photosynthesis of Atropa belladonna L. leaves, with superoxide (SOD), peroxidase (POD), and catalase (CAT) activities increasing to different degrees; malondialdehyde (MDA) content decreased significantly; nitrate nitrogen, soluble protein, and free amino acid content and nitrate reductase (NR), glutamine synthetase (GS), and glutamate dehydrogenase (GDH) activities increased significantly. The contents of precursor amino acids (ornithine and arginine) and tropane alkaloids (scopolamine and scopolamine) in the secondary metabolic pathway of Atropa belladonna L. also significantly increased. Our results indicated that 100 mmol/L salt stress treatment inhibited the growth of belladonna, and 20 mmol/L betaine treatment could alleviate this damage.

     

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