Abstract:
In this study,
Aconitum carmichaelii Debx. and rhizosphere soil from different areas were studied. The changes of endophytic microorganisms, soil microorganisms, alkaloid content and soil element factors of
Aconitum carmichaelii were studied by illuminahiseq high-throughput sequencing technology and high performance liquid chromatography. The results showed that: the contents of benzoylneaconitine, benzoylaconitine and monoester alkaloids were positively correlated with
Acinetobacter abundance, soil pH and Al
3+ content,while negatively with
Aspergillus abundance. The content of hypoaconitine was positively correlated with
Bradyrhizobium abundance. However, the other two alkaloids (benzoylsubaconitine and hypaconitine) accumulations were negatively correlated with
Athelia and
Fusarium abundances. The results show that: changing the abundances of the key microorganism (such as
Bradyrhizobium, Acinetobacter, Athelia, Fusarium and
Aspergillus), and improving the Al
3+ contents and pH in rhizosphere soil probably increase the contents of several alkaloids in
Aconitum carmichaelii field management programs.