山金柑实生后代四倍体发掘及形态和代谢评价

doi:10.11913/PSJ.2095-0837.2022.10047

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摘要

以多胚系山金柑（Fortunella hindsii Swingle）为材料，采用“观根辨叶看油胞”形态初选法，从1289株实生后代筛选出疑似四倍体（双二倍体）8株，流式细胞仪检测和SSR分子鉴定表明它们均为同源四倍体，初选准确率100%，群体自然发生率0.62%。对其形态和初生代谢物进行检测，结果显示：山金柑四倍体株高、茎粗、节间数、节间长、气孔密度均显著低于二倍体，而叶片厚度、气孔大小显著高于二倍体；GC-MS分析鉴定到24种初生代谢物，四倍体叶片奎宁酸含量显著高于二倍体，肌醇、4-氨基丁酸和1-棕榈酸单甘油酯含量显著低于二倍体。

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	张成磊
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	解凯东
	夏强明
	伍小萌
	郭文武

关键词 ：山金柑, 多倍体, 流式细胞仪, SSR分子标记, 初生代谢

Abstract：

To explore tetraploid seedlings from polyembryonic Hongkong kumquat (Fortunella hindsii Swingle), we screened eight putative tetraploids (doubled diploids) from 1289 seedlings based on root, leaf, and oil gland morphology. Flow cytometry and molecular identification showed that these tetraploids were all autotetraploids, with a screening accuracy of 100% and natural occurrence rate of 0.62%. Compared with diploids, the tetraploids of Hongkong kumquat showed significantly lower plant height, stem diameter, internode number, internode length, and stomatal density, but higher leaf thickness and stomatal size. In addition, leaf primary metabolites between the tetraploids and corresponding diploids were analyzed by gas chromatography-mass spectrometry (GC-MS). Among the 24 detected primary metabolites, the content of quininic acid in the tetraploid leaves was significantly higher than that in the diploid leaves, while the contents of inositol, 4-aminobutyric acid, and 1-monopalmitin were significantly lower.

Key words： Fortunella hindsii Polyploidy Flow cytometry Simple sequence repeat Primary metabolites

收稿日期: 2021-09-13

ZTFLH:

S666.1

基金资助:

国家自然科学基金（32172525）；广东省科技计划（2018B020202009）；湖北省科技计划（2020BBA036，2021DFE016）。

通讯作者: 郭文武,guoww@mail.hzau.edu.cn E-mail: guoww@mail.hzau.edu.cn

作者简介: 张成磊(1987-),男,硕士,实验师,研究方向为柑橘种质资源发掘与利用(E-mail:zcl_mail@yeah.net)。

引用本文:

张成磊, 周锐, 谢善鹏, 解凯东, 夏强明, 伍小萌, 郭文武. 山金柑实生后代四倍体发掘及形态和代谢评价[J]. 植物科学学报, 2022, 40(1): 47-53. Zhang Cheng-Lei, Zhou Rui, Xie Shan-Peng, Xie Kai-Dong, Xia Qiang-Ming, Wu Xiao-Meng, Guo Wen-Wu. Exploration and evaluation of morphological traits and primary metabolites of tetraploid seedlings from Hongkong kumquat (Fortunella hindsii Swingle). Plant Science Journal, 2022, 40(1): 47-53.

链接本文:

http://www.plantscience.cn/CN/10.11913/PSJ.2095-0837.2022.10047 或 http://www.plantscience.cn/CN/Y2022/V40/I1/47

[1] 邓秀新. 中国柑橘品种[M]. 北京:中国农业出版社, 2008.
[2] 郭文武, 叶俊丽, 邓秀新. 新中国果树科学研究70年——柑橘[J]. 果树学报, 2019, 36(10):1264-1272. Guo WW, Ye JL, Deng XX. Fruit scientific research in New China in the past 70 years:Citrus[J]. Journal of Fruit Science, 2019, 36(10):1264-1272.
[3] Zhu CQ, Zheng XJ, Huang Y, Ye JL, Chen P, et al. Genome sequencing and CRISPR/Cas9 gene editing of an early flowering Mini-Citrus (Fortunella hindsii)[J]. Plant Biotechnol J, 2019, 17:2199-2210.
[4] 周锐, 解凯东, 王伟, 彭珺, 谢善鹏, 等. 依据多倍体形态特征快速高效发掘柑橘四倍体[J]. 园艺学报, 2020, 47(12):2451-2458. Zhou R, Xie KD, Wang W, Peng J, Xie SP, et al. Efficient identification of tetraploid plants from seedling populations of apomictic citrus genotypes based on morphological characteristics[J]. Acta Horticulturae Sinica, 2020, 47(12):2451-2458.
[5] 张斯淇, 徐强, 邓秀新. 无融合生殖与柑橘多胚现象的研究进展[J]. 植物科学学报, 2014, 32(1):88-96. Zhang SQ, Xu Q, Deng XX. Advances in apomixis and polyembryony research in Citrus plants[J]. Plant Science Journal, 2014, 32(1):88-96.
[6] 梁武军, 解凯东, 郭大勇, 谢宗周, 伊华林, 郭文武. 10个柑橘砧木类型同源四倍体的发掘与SSR鉴定[J]. 果树学报, 2014, 31(1):1-6. Liang WJ, Xie KD, Guo DY, Xie ZZ, Yi HL, Guo WW. Spontaneous generation and SSR molecular characterization of autotetraploids in ten citrus rootstocks[J]. Journal of Fruit Science, 2014, 31(1):1-6.
[7] 彭滢, 李晓妍, 肖璇. 柑橘多胚性砧木枳橙同源四倍体的发掘与SSR鉴定[J]. 分子植物育种, 2020, 18(4):1211-1215. Peng Y, Li XY, Xiao X. Excavation and SSR identification of autotetraploids in citrus polyembryonic rootstock citrange[J]. Molecular Plant Breeding, 2020, 18(4):1211-1215.
[8] 蒋景龙, 阳妮, 李丽, 秦公伟, 邓家锐, 等. 衢州香橙四倍体种质发掘及形态特征性评价[J]. 果树学报, 2021, 38(5):655-663. Jiang JL, Yang N, Li L, Qin GW, Deng JR, et al. Identification and characterization of tetraploids from seedlings of Citrus junos ‘Quzhou xiangcheng’[J]. Journal of Fruit Science, 2021, 38(5):655-663.
[9] 梁武军, 解凯东, 郭大勇, 谢宗周, 徐强, 等. 柑橘10个品种实生后代多倍体的发掘及SSR鉴定[J]. 园艺学报, 2014, 41(3):409-416. Liang WJ, Xie KD, Guo DY, Xie ZZ, Xu Q, et al. Spontaneous generation and SSR characterization of polyploids from ten citrus cultivars[J]. Acta Horticulturae Sinica, 2014, 41(3):409-416.
[10] 解凯东, 彭珺, 袁东亚, 强瑞瑞, 谢善鹏, 等. 以本地早橘和槾橘为母本倍性杂交创制柑橘三倍体[J]. 中国农业科学, 2020, 53(23):4961-4968. Xie KD, Peng J, Yuan DY, Qiang RR, Xie SP, et al. Production of citrus triploids based on interploidy crossing with Bendizao and Man tangerines as female parents[J]. Scientia Agricultura Sinica, 2020, 53(23):4961-4968.
[11] 夏强明. 基于2n雌配子有性群体定位柑橘着丝粒及其序列特征分析[D]. 武汉:华中农业大学, 2020:23.
[12] Cheng YJ, Guo WW, Yi HL, Pang XM, Deng XX. An efficient protocol for genomic DNA extraction from Citrus species[J]. Plant Mol Biol Rep, 2003, 21(2):177-178.
[13] Xu Q, Chen LL, Ruan XA, Chen DJ, Zhu AD, et al. The draft genome of sweet orange (Citrus sinensis)[J]. Nat Genet, 2013, 45(1):59-66.
[14] Luro FL, Costantino G, Terol J, Argout X, Allario T, et al. Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping[J]. BMC Genomics, 2008, 9:287.
[15] García-lor A, Luro F, Navarro L, Ollitrault P. Comparative use of InDel and SSR markers in deciphering the interspecific structure of cultivated citrus genetic diversity:a perspective for genetic association studies[J]. Mol Genet Genomics, 2012, 287(1):77-94.
[16] Aleza P, Froelicher Y, Schwarz S, Agustí M, Hernández M, et al. Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment[J]. Ann Bot, 2011, 108(1):37-50.
[17] Cuenca J, Froelicher Y, Aleza P, Juárez J, Navarro L, Ollitrault P. Multilocus half-tetrad analysis and centromere mapping in citrus:evidence of SDR mechanism for 2n megagametophyte production and partial chiasma interference in mandarin cv ‘Fortune’[J]. Heredity, 2011, 107(5):462-470.
[18] 宋鑫, 谭丰全, 张苗, 蔡元康, 郭大勇, 等. ‘纽荷尔’脐橙与‘尤力克’柠檬种间体细胞杂种的代谢特征分析[J]. 园艺学报, 2019, 46(1):37-46. Song X, Tan FQ, Zhang M, Cai YK, Guo DY, et al. Metabolic characteristics of interspecific allotetraploid somatic hybrid between ‘Newhall’ navel orange and ‘Eureka’ lemon[J]. Acta Horticulturae Sinica, 2019, 46(1):3746.
[19] Neilson EH, Goodger JQ, Woodrow IE, Moller BL. Plant chemical defense:at what cost?[J]. Trends Plant Sci, 2013, 18(5):250-258.
[20] Tan FQ, Tu H, Liang WJ, Long JM, Wu XM, et al. Comparative metabolic and transcriptional analysis of a doubled diploid and its diploid citrus rootstock (C. junos cv. Ziyang xiangcheng) suggests its potential value for stress resistance improvement[J]. BMC Plant Biol, 2015, 15(1):89.