Abstract:
The molecular characteristics and expression patterns of glutathione peroxidase genes (
GPX) in moso bamboo (
Phyllostachys edulis (Carriere) J. Houzeau) were analyzed using bioinformatics and molecular biology. A total of nine
GPX genes were identified from the genome of moso bamboo (
PeGPX1-PeGPX9), which all contained six exons and five introns. The lengths of proteins encoded by
PeGPXs ranged from 168 to 235 aa, with relative molecular weights of 18.41 to 25.54 kD and isoelectric points of 5.88 to 9.48. Subcellular localization analysis predicted that all PeGPXs were localized on the chloroplast, except for PeGPX5 on mitochondria. A variety of stress and hormone-related
cis-acting elements were found in the promoters of
PeGPXs. Quantitative real-time polymerase chain reaction (qRT-PCR) showed that the expression levels of
PeGPXs in the leaves of moso bamboo were distinctly affected by high light, low temperature, GA
3, NAA, and MeJA treatments.