Extraction and RAPD Analysis of Mitochondrial DNA of Cytoplasmic Male Sterile(CMS) Line in Broccoli
ZHU Yu-Ying1,2, YANG Xiao-Feng1,2, HOU Rui-Xian1,2, LI Shi-Jun3, WU Qin-Sheng3
1. Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;2. Shanghai Key Lab.of Protected Horticultural Technology, Shanghai 201106, China;3. Department of Horticulture, Nanjing Agri.University, Nanjing 210095, China
Abstract：DNA from the mitochondrion(mtDNA) of CMS line BC7-19 and its maintaner of broccoli(Brassica oleracea L.var.italica Plenck.) was extracted by molecular bio-technique,and was marked(using) RAPD reaction.The result showed that the purity of the extracted mtDNA was high by sepharose(electrophoresis).And the result also showed that a special fragment,BMS1000,with about(1000) bp amplificated by primer S66,was peculiar for the mtDNA of male sterile line by RAPD reaction of 40 primers,and the special fragment was initially considered to be relevant to male sterility after sequence analysis.
朱玉英;杨晓锋;侯瑞贤;李式军;吴琴生. 青花菜细胞质雄性不育系线粒体DNA的提取与RAPD分析[J]. 植物科学学报, 2006, 24(6): 505-508.
ZHU Yu-Ying;YANG Xiao-Feng;HOU Rui-Xian;LI Shi-Jun;WU Qin-Sheng. Extraction and RAPD Analysis of Mitochondrial DNA of Cytoplasmic Male Sterile(CMS) Line in Broccoli. , 2006, 24(6): 505-508.
2006, Vol. 24 
