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阎华, 周嫦, 杨弘远. 向日葵未受精胚珠培养中各种影响因素的实验研究[J]. 植物科学学报, 1988, 6(4): 319-326.
引用本文: 阎华, 周嫦, 杨弘远. 向日葵未受精胚珠培养中各种影响因素的实验研究[J]. 植物科学学报, 1988, 6(4): 319-326.
Yan Hua, Zhou Chang, Yang Hongyuan. EXPERIMENTAL STUDIES ON SEVERAL FACTORS AFFECTING UNFERTILIZED OVULE CULTURE IN SUNFLOWER[J]. Plant Science Journal, 1988, 6(4): 319-326.
Citation: Yan Hua, Zhou Chang, Yang Hongyuan. EXPERIMENTAL STUDIES ON SEVERAL FACTORS AFFECTING UNFERTILIZED OVULE CULTURE IN SUNFLOWER[J]. Plant Science Journal, 1988, 6(4): 319-326.

向日葵未受精胚珠培养中各种影响因素的实验研究

EXPERIMENTAL STUDIES ON SEVERAL FACTORS AFFECTING UNFERTILIZED OVULE CULTURE IN SUNFLOWER

  • 摘要: 对向日葵未受精胚珠培养中的若干影响因素进行了比较研究,着重分析它们对孤雌生殖胚状体、珠被绒毡层胚状体和珠被愈伤组织等三种产物的不同作用。研究的因素包括品种特性、基本培养基、外植体类型、胚囊发育时期、培养方式、浅层与深层培养、胚珠密度以及光照条件,它们对培养结果各有不同程度的影响。调节上述因素,可以促进孤雌生殖和控制体细胞增生。

     

    Abstract: In sunflower ovule culture, there are two kinds of products; the gynogenetic embryoid, somatic proliferation including endothelial embryoid and the integumental callus. It is very important to regulate the balancne between them in favor of the former. We have carried out a large-scale design of experiments. The factors studied included: donor cultivars, basic media, kind of explents, embryo sac stage, culture on solid or liquid medium, shallow culture and ovule-density culture, and illumination condition, etc. Each factor could influence each kind of the derivatives differently. By regulation of various factors we were able to enhance induction-frequency of gynogenesis and meanwhile eliminate somatic derivatives. A suitable procedure in sunflower ovule culture concluded in this paper is to choose high-responsive cultivars; pretreat the inflorescence at 4℃ for 24—48 hours; pick out ovules from the florets 1—3 days before anthesis and inoculate them on N6 medium(solid or liquid) supplemented with 12% sucrose, 100mg/l inositot, but free of exogenous hormone, keep the culture in approx.27℃ and darkness for about 30 days; dissect the enlarged ovules and take out, if any induced, the gynogenetic embryoids; transfer the embryoids onto adequate medium for regeneration.

     

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