Abstract: Fluorescence in situ hybridization(FISH) was carried out on mitotic chromosomes of Zea diploperennis(DP),maize inbred lines F107,GB57 and their hybrids using knob-associated tandem repeats,180-bp repeats and TR-1 elements,together with 45S rDNA as probes.The distribution patterns of the three repeated sequences were analyzed on parental chromosomes.The hybrids of maize and DP were(identified by) FISH.According to the hybridization signals on chromosome positions,the origin of foreign idioplasm was confirmed.The application using knob-associated tandem repeats as excellent chromosomal markers on detecting the integration of foreign chromosome and the chromosomal activity at meiosis was also discussed.