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温颖群. 椰子幼花序离体培养的器官发生[J]. 植物科学学报, 1997, 15(1): 39-42.
引用本文: 温颖群. 椰子幼花序离体培养的器官发生[J]. 植物科学学报, 1997, 15(1): 39-42.
Wen Yingqun. ORGANOGENESIS FROM IN VITRO CULTURE OF YOUNG COCONUT INFLORESCENCE[J]. Plant Science Journal, 1997, 15(1): 39-42.
Citation: Wen Yingqun. ORGANOGENESIS FROM IN VITRO CULTURE OF YOUNG COCONUT INFLORESCENCE[J]. Plant Science Journal, 1997, 15(1): 39-42.

椰子幼花序离体培养的器官发生

ORGANOGENESIS FROM IN VITRO CULTURE OF YOUNG COCONUT INFLORESCENCE

  • 摘要: 椰子幼花序在改良的MS附加高浓度的NAA与2,4-D的培养基中产生愈伤组织,在逐渐降低NAA和2,4-D浓度的过程中,愈伤组织产生结构紧密的结节组织。结节组织内有输导组织、分生细胞团、根原基及不同发育阶段的胚状体,有些进而发育成正常的根和绿色芽状物。同时,某些幼花枝的基部直接长出绿芽,经继续培养形成具有正常很、叶结构的完整植株,但茎尖无顶端分生组织,无叶原基,有的发育成花枝,甚至在顶部长出几朵发育不正常的小花。

     

    Abstract: Callus was induced in the modified MShasal medium with high concentration of NAAand 2,4-Dfrom young inflorescence of coconut (Cocos nucifera L.).During the callus subculture with gradually reduced concentration of NAAand 2,4-D,it developed into compact nodular tissue.There were conducting tissue,meristematic cell clumps,root primordia and embryoids at different developing stage in the nodular tissue.The normal roots and green buds were developed subsequently from some of the nodular tissue.At the same time,some green buds grew out directly from the base of the young flower branches, and finally became the plantlets with normal roots and leaves,but without apical meristem and leaf primordia.Moreover,some of the stem apex developed into flower branches and even showed several small abnormal flowers.

     

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