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赵洁, 程井辰, 熊进, 何亚文, 杨晓红. 石刁柏愈伤组织根和芽分化过程中POD和SOD活性的变化[J]. 植物科学学报, 1997, 15(1): 49-53.
引用本文: 赵洁, 程井辰, 熊进, 何亚文, 杨晓红. 石刁柏愈伤组织根和芽分化过程中POD和SOD活性的变化[J]. 植物科学学报, 1997, 15(1): 49-53.
Zhao Jie, Cheng Jingchen, Xiong Jin, He Yawen, Yang Xiaohong. CHANGES OF POD AND SOD ACTIVITIES DURING ADVENTITIOUS ROOT AND BUD DIFFERENTIATION IN ASPARAGUS OFFICINALIS L.[J]. Plant Science Journal, 1997, 15(1): 49-53.
Citation: Zhao Jie, Cheng Jingchen, Xiong Jin, He Yawen, Yang Xiaohong. CHANGES OF POD AND SOD ACTIVITIES DURING ADVENTITIOUS ROOT AND BUD DIFFERENTIATION IN ASPARAGUS OFFICINALIS L.[J]. Plant Science Journal, 1997, 15(1): 49-53.

石刁柏愈伤组织根和芽分化过程中POD和SOD活性的变化

CHANGES OF POD AND SOD ACTIVITIES DURING ADVENTITIOUS ROOT AND BUD DIFFERENTIATION IN ASPARAGUS OFFICINALIS L.

  • 摘要: 研究了石刁柏愈伤组织根和芽分化过程中可溶性蛋白质含量、过氧化物酶(POD)、超氧物歧化酶(SOD)和非特异性酯酶(NSE)活性变化的规律。结果表明:①在根和芽分化过程中,可溶性蛋白质含量增加,第8~10d增至高峰时,分别为愈伤组织的1.98倍(根)和2.19倍(芽);随着根和芽进一步生长,可溶性蛋白质含量下降。②在根(第10d)和芽(第8d)形成时,POD活性达到最高水平,分别为1.92和2.52△A.mg-1prot·min-1.根和芽分化过程中POD活性变化规律基本相似。③在根和芽分化过程中SOD均出现2个活性峰,前者分别为第2d和第10d,后者分别为第4d和第8d。其中第10d和第8d的峰值与根和芽形成有关。④根分化过程中NSE活性变化与POD相似,而芽分化过程中NSE活性出现2个峰值,分别在第6d和第10d。

     

    Abstract: The changes of soluble protein content,peroxidase (POD),superoxlde dismutase (SOD), and non-specific esterase (NSE)activities during adventitious root and bud differentiation in Asparagus officinalis(L.)were reported.(1) The soluble protein content increased in the course of formation of root and bud.The peak values were 1.98 (root)and 2.19 (bud)times as much as callus, respectively.The soluble protein content decreased during the following growth of root and bud.(2) The peak value of the PODactivity was the highest at the formation of root (in 10th day)and bud(in 8th day), 1.92 and 2.52 △A?mg-1 prot?min-1, respectively.The change regularity of POD activity was similar in the course of root and bud differentiation.(3)The SODactivity presented two peaks,in 2nd day and 10th day during the differentiation of root,in 4th day and 8thday during the differentiation of bud, respectively.The peak values of SODactivity in 10th day and sib day had relation with the formation of root and bud, (4) The change of NSEactivity was similar to that of PODactivity during the differentiation of root.The NSE activity presented two peaks,in 6th day and 10th day,respectively,during the differentiation of bud.

     

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