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陈凡, 陈海芳, 傅俊. 紫藤凝集素的分离纯化及理化性质研究[J]. 植物科学学报, 1994, 12(3): 270-276.
引用本文: 陈凡, 陈海芳, 傅俊. 紫藤凝集素的分离纯化及理化性质研究[J]. 植物科学学报, 1994, 12(3): 270-276.
Chen Fan, Chen Haifang, Fu Jun. PURIFICATION AND PHYSICHEMICAL CHARACTERIZATION OF A LECTIN FROM WISTARIA SINENSIS SWEET[J]. Plant Science Journal, 1994, 12(3): 270-276.
Citation: Chen Fan, Chen Haifang, Fu Jun. PURIFICATION AND PHYSICHEMICAL CHARACTERIZATION OF A LECTIN FROM WISTARIA SINENSIS SWEET[J]. Plant Science Journal, 1994, 12(3): 270-276.

紫藤凝集素的分离纯化及理化性质研究

PURIFICATION AND PHYSICHEMICAL CHARACTERIZATION OF A LECTIN FROM WISTARIA SINENSIS SWEET

  • 摘要: 用常规方法处理的DEAE离子交换纤维素柱,通过线性离子强度梯度洗脱,从紫藤种子的蛋白粗提液中得到一定纯度的紫藤凝集素。纯化的凝集素凝集兔红血球的比活提高40倍,总活力回收率为19.2%.紫藤凝集素的分子量经PAGE鉴定为205kd,是由两种亚基构成的四聚体,这两种亚基各有2个,分子量经SDS-PAGE鉴定分别为77600d和25100d.紫藤凝集素是一种糖蛋白,等电点约为4.60.它可凝集人的各种血型和数种动物血,但无专一性。它的凝集活性可被数种糖抑制,其中N-乙酰-D-半乳糖胺最高。氨基酸组成分析表明紫藤凝集素谷氨酸、天冬氨酸含量较高,而胱氨酸、脯氨酸、酪氨酸含量甚微。

     

    Abstract: A lectin from Wistaria sinensis has been purified respectively from the solution of crude extract by DEAE-C ion-exchange chromatographic column. The DEAE-C ion-exchange chromatographic column has been treated by ordinary methods and the lectin is eluted from the column by a linear ionie strength gradient. The hemagglutination activity of the purified lectin showed an activity increase of 40 fold for rabbit erythrocytes.The recovery of total activity was 19.2%.The molecular weight of the lectin from Wistaria sinensis is 205kd as determined by PAGE.The lectin molecule is a tetramer which consists of two kind of subunits and each kind of subunits has two.The molecular weights of these subunits are 77600d and 25100d as determined by SDS PAGE.The lectin from Wistaria sinensis a kind of glycoprotein.The isoelectric point of the lectin is 4.60.The lectin can agglutinate the erythocytes of each human blood group and of some animals, but it is nonspecific.The hemagglutination activity is inhibited by many saccharidesand it can be inhibited mostly by N-acetyl-D-galactosamine.Amino acid analysis shows that the lectin molecule contained relatively more Glu,Asp and less Cys-Cys, Pro, Tyr.

     

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