Abstract:Aichi Hakusai(C1),which was a Chinese cabbage cultivar(Brassicarapa ssp.pekinensis(L.)Olsson)introduced from Japan,was used as plant material in this experiment.Investigations were carried out on histological and physiological changes during the process of in vitro shoot regeneration from cotyledonary explants.Histological observations showed that,(adventitious)shoots were induced through the pathway of direct organogenesis during the process of explants culture.Before adventitious shoots initiated,soluble protein content and activities of POD and SOD showed the trend to increase.As the cells were dedifferentiated,metabolism activity of cells gradually became stronger;enzymes activity and the soluble protein content were elevated.These results demonstrated that,morphological changes were strongly associated with physiological changes during the process of adventitious shoot induction.Addition of AgNO3 to the medium increased the activity of enzymes and promoted the differentiation percentage of(adventitious)shoots.
樊明琴;朱月林;朱丽华. 结球白菜离体子叶不定芽再生过程中的组织学及生理变化[J]. 植物科学学报, 2005, 23(2): 138-143.
FAN Ming-Qin;ZHU Yue-Lin;ZHU Li-Hua. Histological and Physiological Changes in the Process of in vitro Shoot Regeneration from Cotyledons of Chinese Cabbage. , 2005, 23(2): 138-143.