Abstract: This paper deals with the taxonomic revision of the genus Arachniodes from Yunnan. Twenty species of the genus Arachniodes from Yunnan are confirmed. Of which, A. festina (Hance) Ching and A. aristata (Forst.) Tindale are new records to Yunnan; Acrorumohra dissecta Ching et Y. T. Hsieh, Arachniodes ailaoshanensis Ching, A. australis Y. T. Hsieh, A. austro-yunnanensis Ching, A. caudata Ching, A. chinensis (Rosenst.) Ching var. cornopteris (Ching) S. K. Wu et H. He, A. chinensis (Rosenst.) Ching var. jinpingensis (Y. T. Hsieh) S. K. Wu et H. He, A. chingii Y. T. Hsieh, A. dayaoensis Y. T. Hsieh, A. futeshanensis Y. T. Hsieh, A. gongshanensis Ching et Y. T. Hsieh, A. guangtongensis Ching, A. lushuiensis Y. T. Hsieh, A. pianmaensis Ching, A. pseudo-aristata (Tagawa) Ohwi, A. pseudo-assamica Ching, A. shuangbaiensis Ching, A. sinoaristata Ching, A. sporadosora (Kunze) Nakaike and A. triangularis Ching are new synonyms. The key to the species, the nomenclatural treatment, including taxonomic notes, the distribution are also provided.