Abstract: The optimum selection of plant regulator concentrations was carried out by means of orthogonal experiment in Chinese gooseberry (Actinidia chinensis)culture in vitro. The results have shown that the changes of Zt concentration have significant effects on the percentage of callus induced, the avarage weight of callus and the avarage number of multiple-shoots, but there was no singnificance in the changes of concentrations of Kt and NAA. To percentage of callus induction, the best combinations of plant regulators were 1.5mg/L Zt+0.05mg/L Kt+0.95mg/L NAA; to avarage number of multiple-shoots and avarage weight of callus, the best combinations of plant regulators were 2.9mg/L Zt+0.05mg/L Kt+1.5mg/L NAA.