Cloning and analysis of chalcone synthase gene FtCHS1 promoter in Fagopyrum tataricum Gaertn.

Through genome walking,a 5' flanking sequence of the chalcone synthase gene,1038 bp in length,was cloned from tartary buckwheat (Fagopyrum tataricum Gaertn).Bioinformatics analysis showed 63.5% of adenine and thymine in the sequence.Four potential transcription starting sites were located at-684-734 bp,-692-742 bp,-920-970 bp,and-929-979 bp,respectively,which not only contained promoter elements such as TATA-Box and CAAT-Box,but also functional elements related to light,low temperature,and hormone responses.In this study,the plant expression vector was further constructed and transformed into Arabidopsis thaliana L.leaves,and results showed that the sequence could drive the expression of the GUS reporter gene,and was thus named as promoter P_FtCHS1.The tartary buckwheat sprouts were then treated by low temperature (4℃) and UV-B light,with P_FtCHS1 shown to respond to the temperature and light treatment,which resulted in a change in FtCHS1 gene expression.