Mining and analysis of the self-incompatibility S gene in Aconitum pendulum N. Busch based on RNA-seq
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Abstract
Two Aconitum pendulum N. Busch cultivars (‘WSYB1’ and ‘WSYY1’) were used as materials for transcriptome sequencing (RNA-seq) using bioinformatics to identify possible style and pollen S genes and analyze their sequence characteristics. Results identified two style S genes (ApSRNase) specifically or highly expressed in the style and two pollen S genes (ApSLF) specifically expressed in pollen. Similar to the sequenced plant Aquilegia coerulea James, this study showed that there was a S-RNase-based self-incompatibility system controlled by S-RNase and SLF in A. pendulum, but no Papaveraceae-type self-incompatibility system controlled by sS and pS.
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