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Lun Han-Ning, Ye Zhong-Ming, Yang Chun-Feng. Comparison of floral nectar extraction, preservation, and detection methods[J]. Plant Science Journal, 2021, 39(2): 201-207. DOI: 10.11913/PSJ.2095-0837.2021.20201
Citation: Lun Han-Ning, Ye Zhong-Ming, Yang Chun-Feng. Comparison of floral nectar extraction, preservation, and detection methods[J]. Plant Science Journal, 2021, 39(2): 201-207. DOI: 10.11913/PSJ.2095-0837.2021.20201

Comparison of floral nectar extraction, preservation, and detection methods

  • Nectar is rich in organic compounds and microelements. However, nectar volume and chemical composition can vary substantially among plant species. Thus, nectar extraction and storage methods may affect component analysis. Based on a systematic review of the current literature, we summarizes six common nectar sampling methods in the field, including micropipettes and microsyringes, microcapillary tubes, filter paper, solution dilution, centrifugation, and aspiration; six nectar storage methods, including refrigeration at 4 ℃, freezing at -20 ℃, ultra-low freezing at -80 ℃, liquid nitrogen storage at -198 ℃, antimicrobial preservation, and filter paper storage; and three nectar characteristic measurement methods, including handheld refractometry, colorimetry, and chromatography. We further discuss the advantages and disadvantages of these methods and their potential application for different plant species. Under appropriate conditions, the most commonly used methods for nectar collection, storage, and analysis are microcapillary tubes, ultra-low freezing at -80 ℃, and chromatography.
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