Isolation of the Promoter Region of a Pollen-specific Gene PSG076 by Inverse-PCR

A pollen-specific gene,PSG076,with unknown function,was isolated from Austrian wheat ‘Ferdinand’.To obtain pollen-specific promoter in wheat genetic engineering,the promoter region of PSG076 gene was isolated by an improved inverse-PCR (IPCR) method.A 1.4 kb promoter sequence was cloned and analyzed by Bioinformatics tools.Some cis-acting elements or enhancers conferred to pollen-specific expression were found,such as AGAAA,GTGA, AGGTCA and AAATGA.This suggested that PSG076 promoter might be a pollen-specific promoter with strong activity.The results also showed that the improved IPCR method was efficient to isolate the flanking unknown sequence,especially in promoter isolation.