cDNA Cloning and Bioinformatic Analysis of the sPPa1 Gene from Picea wilsonii

The full-length cDNA sequence of the PPa1 gene was obtained by the RACE method based on the cDNA library of Picea wilsonii.Bioinformatic analysis was used to predict the physicochemical properties,hydrophobicity,secondary structure,and tertiary structure of PwPPa1.Multiple sequences alignment and phylogenetic trees were also constructed to predict the conserved domain and genetic relationship with other species.The RT-qPCR assays were used to identify the tissue expression level of PPa1 in Picea wilsonii.The results showed that PwPPa1 consisted of 216 amino acids.The molecular weight was 24.55 kD and theoretical PI was 5.83.The PPa1 was a hydrophilic protein and the secondary structure of PPa1 was mainly composed of alpha helix,random coil,and extended strand.The expression level of PPa1 was highest in the pollen.This research provides the foundation for further studies on the functions of PwPPa1.