Extraction and Measurement of Total Protein and Amino Acids,Analysis the Identification Result of Antifreeze Protein in Ammopiptanthus nanus

Total protein of the leaves of Ammopiptanthus nanus was extracted,the buffer solution system was established which can obtain max leaves proteins from A.nanus.All protein groups were isolated by DEAE₅₂-C,whose molecular weights(14-90 kD)and purities were measured by SDS-PAG.The results of relative percentage of amino acids revealed the genetic consistency of the different species.Four protein groups were identified as antifreeze proteins whose thermal hysteresis activities(THA)were 0.4℃,0.33℃,0.48℃ and 0.15℃,respectively.This was evidences of discovery of antifreeze proteins in A.nanus.