Cytological Observation of Anther Development between the Male Sterile Line and Its Maintainer Line in Non-heading Chinese Cabbage

Buds selected at different developmental stages of a new germplasm sterile male of non-heading Chinese cabbage and its maintainer lines were studied.Anthers were collected,made into paraffin sections,stained,and observed by electron microscopy.Results show that non-heading Chinese cabbage sterile male lines and maintainer lines exhibited quite different anther development.Male sterile lines in relation to anther development were inhibited at the stage of archesporial cell,produced only 1-3 anther sacs,and formed normal sporogenous cells.The cells gradually disintegrated,formated cavity,and finally shrunk inward.The maintainer archesporial cells formed normal anther sacs,formed sporogenous cell,and ultimately formed anthers with normal pollen.