Embryogenesis of Isolated-microspore in Cabbage(Brassica oleracea var.capitata)

Factors affecting microspore embryogenesis in cabbage was intensively investigated(using) cultivar,‘Qiangxia’.Results showed that the optimum period for donor flower buds collection was before full bloom of the plants.Embryoids could develop from microspores of uninucleate stage to binucleate stage.Medium with 17% sucrose at the first days of culture was favorable for the vitality of microspores,but medium with 14% sucrose was favorable for the embryogenesis after 3 days of culture.It was found that the embryogenesis ability was greatly improved by adding low sucrose concentration(11%) medium into the medium with high sucrose concentration(17%) after 3 days of culture.In this case,the embryogenesis frequency increased 282.4% and 126.1% respectively compared with microspores cultured consistently in medium with 14% sucrose or changing the medium completely.